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rabbit polyclonal lig3 antibody  (ABclonal Biotechnology)


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    Structured Review

    ABclonal Biotechnology rabbit polyclonal lig3 antibody
    Rabbit Polyclonal Lig3 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+lig3+antibody/anti+lig3/pm37315345-156-6-10
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal lig3 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Binding Assay:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Luciferase:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Expressing:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Quantitative RT-PCR:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Western Blot:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Knockdown:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Two Tailed Test:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Cell Culture:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Over Expression:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Microinjection:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Plasmid Preparation:

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla.
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 ◦C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell

    Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
    Article Snippet: membranes containing proteins were blocked with QuickBlock buffer (Beyotime, China) for 1 h at room temperature. .. Afterward, the membranes were blotted with rabbit polyclonal Lig3 (1:1000, ABclonal, China) and mouse monoclonal horseradish peroxidase (HRP)-conjugated GAPDH (1:5000, Proteintech, USA) primary antibodies at 4 °C overnight. .. After washing the membranes three times with PBST, they were hybridized with goat anti-rabbit IgG-HRP (1:3000, Cell



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    Figure 4. Replication and BER/SSBR proteins detected by iPOND-PRM. Bars represent normalized peptide peak areas of replisome enriched (light gray), chromatin enriched (dark gray) and proteins with no enrichment (mid gray). Mean ± SD are shown for PCNA, POL, POLε, FEN1, LIG1, <t>LIG3,</t> XRCC1, APE1, MPG, NEIL1, NTH1 (all n = 5), NEIL3 (n = 4) and MYH (n = 3). For UNG2, POL and NEIL2, each biological replicate is illustrated. Statistical significance was calculated using a two-sided paired Student’s t-test, where *: P < 0.05, **: P < 0.01, n.s: not significant. The FA concentration used for iPOND was 0.25%.
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    Image Search Results


    Figure 4. Replication and BER/SSBR proteins detected by iPOND-PRM. Bars represent normalized peptide peak areas of replisome enriched (light gray), chromatin enriched (dark gray) and proteins with no enrichment (mid gray). Mean ± SD are shown for PCNA, POL, POLε, FEN1, LIG1, LIG3, XRCC1, APE1, MPG, NEIL1, NTH1 (all n = 5), NEIL3 (n = 4) and MYH (n = 3). For UNG2, POL and NEIL2, each biological replicate is illustrated. Statistical significance was calculated using a two-sided paired Student’s t-test, where *: P < 0.05, **: P < 0.01, n.s: not significant. The FA concentration used for iPOND was 0.25%.

    Journal: Nucleic acids research

    Article Title: Monitoring of the spatial and temporal dynamics of BER/SSBR pathway proteins, including MYH, UNG2, MPG, NTH1 and NEIL1-3, during DNA replication.

    doi: 10.1093/nar/gkx476

    Figure Lengend Snippet: Figure 4. Replication and BER/SSBR proteins detected by iPOND-PRM. Bars represent normalized peptide peak areas of replisome enriched (light gray), chromatin enriched (dark gray) and proteins with no enrichment (mid gray). Mean ± SD are shown for PCNA, POL, POLε, FEN1, LIG1, LIG3, XRCC1, APE1, MPG, NEIL1, NTH1 (all n = 5), NEIL3 (n = 4) and MYH (n = 3). For UNG2, POL and NEIL2, each biological replicate is illustrated. Statistical significance was calculated using a two-sided paired Student’s t-test, where *: P < 0.05, **: P < 0.01, n.s: not significant. The FA concentration used for iPOND was 0.25%.

    Article Snippet: Antibodies against POL (ab3181), POL (ab10362, Abcam), XRCC1 (ab1838), LIG1 (ab615), LIG3 (ab587), H3 (ab1791), PCNA (SC-56, Santa-Cruz Biotechnology), FEN1 (A300-256, Bethyl Laboratories) and -UNG2 (TA503755, OriGene) were used.

    Techniques: Concentration Assay